transformed gene meaning in English
转化基因
Examples
- On the development and its safety management of the agricultural transforming gene biological products
转基因植物中的标记基因 - Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively , we can obtain psc and pac . tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac . , then integrated into puc18 and puc19 , by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70 . for the sake of better screening and examination of transformed gene , we cut plasmid 650 , plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes , then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651 . corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis
分别将从psc 、 pac回收纯化的hsp70正、反义cdna与绒毡层特异表达启动子ta29及nos终止子定向连接，然后插入到puc18 、 puc19中，构建成花药特异表达的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分别称作650和651质粒。为了更好地对转化子进行筛选和检测，用hind和ecor分别对650 、 651及3301质粒(含gus报告基因和bar筛选标记基因)进行酶切，将从650和651回收纯化的目的片段与3301质粒进行连接，再对重组子进行平板筛选和酶切分析确定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301质粒中，构建成3301 + 650和3301 + 651表达载体。